渲染靶标:SSR
Render Timestamp:
4/7/2025, 2:18:49 AM EDT
4/7/2025, 6:18:49 AM UTC
Commit: 461ca8d8fe5b1efd4c01fc87e5b5eb592e2d154a
XML generation date: 2025-03-07 13:16:08.520
Product last modified at: 2025-03-21T20:30:08.976Z
1% for the Planet 标识
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

SDHB (E3H9Z) XP® Rabbit mAb #92649

Filter:
  • WB
  • IP
  • IHC
  • IF
  • F
Western Blotting Image 1: SDHB (E3H9Z) XP® Rabbit mAb
Western blot analysis of extracts from various cell lines and tissues using SDHB (E3H9Z) XP® Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). *The antibody detects a 120 kDa protein of unknown identity in some cell extracts.

To Purchase # 92649

Supporting Data

REACTIVITY H M R Mk
SENSITIVITY Endogenous
MW (kDa) 26
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
  • IHC-Immunohistochemistry 
  • IF-Immunofluorescence 
  • F-Flow Cytometry 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Mk-Monkey 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:200
Immunohistochemistry (Paraffin) 1:50 - 1:200
Immunofluorescence (Immunocytochemistry) 1:100 - 1:200
Flow Cytometry (Fixed/Permeabilized) 1:100 - 1:200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

SDHB (E3H9Z) XP® Rabbit mAb recognizes endogenous levels of total SDHB protein. The antibody detects a 120 kDa protein of unknown identity in some cell extracts.

Species Reactivity:

Human, Mouse, Rat, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly208 of human SDHB protein.

Background

Succinate dehydrogenase (SDH), also known as Complex II or succinate:quinone oxidoreductase, is a key component of the citric acid cycle and the electron transport chain (1). Specifically, it is involved in the oxidation of succinate (2). SDH consists of four subunits: SDHA, SDHB, SDHC, and SDHD (3). Research studies have shown that defects in SDHA cause complex II deficiency (2). In addition, investigators have observed reduction of SDHA in the striatum of patients with Huntington’s disease (3), and reduction of SDHB, SDHC, and SDHD in paragangliomas and phenochromocytomas (4,5).
For Research Use Only. Not For Use In Diagnostic Procedures.
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