R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
SCARB1 (E9H4F) Rabbit mAb #90332
Filter:
- WB
- IP
- IHC
- IF
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 80 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunohistochemistry (Paraffin) | 1:150 - 1:600 |
| Immunofluorescence (Frozen) | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
SCARB1 (E9H4F) Rabbit mAb recognizes endogenous levels of total SCARB1 protein. Non-specific staining was observed in normal human small intestine by immunohistochemistry.
Species Reactivity:
Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile487 of mouse SCARB1 protein.
Background
Scavenger receptor class B member 1 (SCARB1, SR-B1) is a multi-ligand cell-surface receptor that facilitates uptake of lipids from cholesterol into cells (1,2). SCARB1 is part of the CD36 scavenger receptor superfamily, which is composed of many proteins with varied structures, expressions, and functions across tissue and cell types (1-3). SCARB1 recognizes a variety of ligands: high density lipoprotein (HDL), phospholipids, phosphatidylserine, and lipoproteins such as lipopolysaccharide (LPS) (2,4,5). SCARB1 knockout mice have elevated HDL levels and increased susceptibility to atherosclerosis when given a high-fat diet, indicating its importance in the regulation of cholesterol homeostasis (6). Additionally, SCARB1 has a role in antigen cross-presentation from bacteria and viruses (1-3). SCARB1 has been reported to be an entry factor for the Hepatitis C virus (7). In endothelial cells and macrophages, SCARB1 has an anti-inflammatory and pro-survival role, which provides protection against atherosclerosis and helps to clear apoptotic cells (8).
- Linton, M.F. et al. (2017) Trends Endocrinol Metab 28, 461-472.
- Hoekstra, M. and Sorci-Thomas, M. (2017) Curr Opin Lipidol 28, 255-260.
- Shen, W.J. et al. (2018) J Lipid Res 59, 1114-1131.
- Acton, S. et al. (1996) Science 271, 518-20.
- Liu, Y. et al. (2008) J Hum Genet 53, 709-717.
- Staršíchová, A. (2023) Cardiovasc Drugs Ther, 1-15.
- Hsu, C.S. et al. (2016) Sci Rep 6, 32303.
- Huby, T. and Le Goff, W. (2022) Curr Opin Lipidol 33, 167-174.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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