R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
SATB2 (E8R8H) Rabbit mAb #39229
Filter:
- WB
- IP
- IHC
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 100 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunohistochemistry (Paraffin) | 1:600 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
SATB2 (E8R8H) Rabbit mAb recognizes endogenous levels of total SATB2 protein. This antibody does not cross-react with SATB1 protein. Non-specific non-nuclear staining was observed in smooth muscle.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val476 of human SATB2 protein.
Background
Special AT-rich binding protein 2 (SATB2) is a close homolog to SATB1 that functions as a transcription factor. It binds to nuclear matrix attachment regions (MARS); regulatory DNA sequences important for chromatin structure. SATB2 was initially identified when bound to the MARS of the immunoglobulin μ gene in pre-B cells, enhancing its expression (1). SATB2 plays a role in osteoblast differentiation by repressing the HoxA2 gene and enhancing the activity of Runx2 and ATF4 (2). SATB2 also plays a role in the developing cerebral cortex by changing chromatin structure surrounding the Ctip2 regulatory regions (3). In erythroid cells, SATB2 activates the γ-globin locus by recruiting PCAF and reordering the chromatin structure (4). Downregulation of SATB2 is linked to colorectal cancer and head and neck squamous carcinomas (5,6).
限制使用
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