SARS-CoV-2 Nucleocapsid Protein (HL344) Rabbit mAb #26369
Filter:
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- IHC
- IF
Supporting Data
| REACTIVITY | Vir |
| SENSITIVITY | Endogenous |
| MW (kDa) | 55 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- Vir-Virus
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| IHC Leica Bond | 1:400 - 1:1600 |
| Immunohistochemistry (Paraffin) | 1:200 - 1:800 |
| Immunofluorescence (Immunocytochemistry) | 1:1600 - 1:6400 |
Storage
Supplied in PBS. This product is stable for 12 months when stored at -20C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present, but will not interfere with antibody performance.
Protocol
Specificity / Sensitivity
SARS-CoV-2 Nucleocapsid Protein (HL344) Rabbit mAb recognizes endogenous levels of total SARS-CoV-2 nucleocapsid (N) protein. The antibody has been confirmed to detect the following SARS-CoV-2 isolates and variants: USA-WA1/2020, Alpha (B.1.1.7), Beta (B.1.351), Gamma (P.1), Delta (B.1.617.2), and Omicron (B.1.1.529). The antibody does not cross-react with nucleocapsid (N) proteins from SARS or MERS coronaviruses.
Species Reactivity:
Virus
Source / Purification
Monoclonal antibody is produced by immunizing animals with a full-length SARS-CoV-2 nucleocapsid (N) recombinant protein.
Background
The cause of the COVID-19 pandemic is a novel and highly pathogenic coronavirus, termed SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2). SARS-CoV-2 is a member of the Coronaviridae family of viruses (1). The genome of SARS-CoV-2 is relatively large and encodes up to 29 open reading frames (ORFs). These include ORF1a and ORF1b (further processed into 16 non-structural proteins), 9 accessory proteins, and 4 canonical structural proteins: spike (S), envelope (E), membrane (M), and nucleocapsid (N) (2). The nucleocapsid (N) protein is a phosphoprotein that plays a critical role in viral assembly (3,4). It binds to the viral genomic RNA, enabling its packaging into the ribonucleoprotein complex (RNP), and is the most abundant protein in the virion (5). Evidence from studies in SARS-CoV-2 and other coronaviruses suggest functions for nucleocapsid (N) protein beyond viral RNA packaging, including roles in viral replication (6) and suppressing host immune responses to viral infection (7).
- Zhou, P. et al. (2020) Nature 579, 270-273.
- Tortorici, M.A. and Veesler, D. (2019) Adv Virus Res 105, 93-116.
- Zengel, J. et al. (2015) J Virol 89, 7338-47.
- Surjit, M. et al. (2005) J Virol 79, 11476-86.
- Peng, Y. et al. (2020) EMBO J, e105938.
- Cong, Y. et al. (2020) J Virol 94, e01925-19. doi: 10.1128/JVI.01925-19.
- Mu, J. et al. (2020) Sci China Life Sci 63, 1-4.
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