SARS-CoV-2 Nucleocapsid Protein (E8R1L) Mouse mAb #33717
Filter:
- WB
- IHC
- IF
Supporting Data
| REACTIVITY | Vir |
| SENSITIVITY | Endogenous |
| MW (kDa) | 55 |
| Source/Isotype | Mouse IgG2a |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- Vir-Virus
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| IHC Leica Bond | 1:800 - 1:3200 |
| Immunohistochemistry (Paraffin) | 1:200 - 1:800 |
| Immunofluorescence (Immunocytochemistry) | 1:400 - 1:1600 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
SARS-CoV-2 Nucleocapsid Protein (E8R1L) Mouse mAb recognizes endogenous levels of total SARS-CoV-2 nucleocapsid (N) protein. This antibody does not cross-react with nucleocapsid proteins from SARS or MERS coronaviruses (assayed by western blot analysis).
Species Reactivity:
Virus
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly30 of SARS-CoV-2 nucleocapsid (N) protein.
Background
The cause of the COVID-19 pandemic is a novel and highly pathogenic coronavirus, termed SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2). SARS-CoV-2 is a member of the Coronaviridae family of viruses (1). The genome of SARS-CoV-2 is relatively large and encodes up to 29 open reading frames (ORFs). These include ORF1a and ORF1b (further processed into 16 non-structural proteins), 9 accessory proteins, and 4 canonical structural proteins: spike (S), envelope (E), membrane (M), and nucleocapsid (N) (2). The nucleocapsid (N) protein is a phosphoprotein that plays a critical role in viral assembly (3,4). It binds to the viral genomic RNA, enabling its packaging into the ribonucleoprotein complex (RNP), and is the most abundant protein in the virion (5). Evidence from studies in SARS-CoV-2 and other coronaviruses suggest functions for nucleocapsid (N) protein beyond viral RNA packaging, including roles in viral replication (6) and suppressing host immune responses to viral infection (7).
- Zhou, P. et al. (2020) Nature 579, 270-273.
- Tortorici, M.A. and Veesler, D. (2019) Adv Virus Res 105, 93-116.
- Zengel, J. et al. (2015) J Virol 89, 7338-47.
- Surjit, M. et al. (2005) J Virol 79, 11476-86.
- Peng, Y. et al. (2020) EMBO J, e105938.
- Cong, Y. et al. (2020) J Virol 94, e01925-19. doi: 10.1128/JVI.01925-19.
- Mu, J. et al. (2020) Sci China Life Sci 63, 1-4.
限制使用
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