SARS-CoV-2 Nsp1 (E3P2T) Rabbit mAb #26000

The cause of the COVID-19 pandemic is a novel and highly pathogenic coronavirus, termed SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2). SARS-CoV-2 is a member of the Coronaviridae family of viruses (1). The genome of SARS-CoV-2 is relatively large and encodes up to 29 open reading frames (ORFs). These include ORF1a and ORF1b (further processed into 16 non-structural proteins), 9 accessory proteins, and 4 canonical structural proteins: spike (S), envelope (E), membrane (M), and nucleocapsid (N) (2). Nsp1 is the first protein to be translated from the viral RNA by the eukaryotic translational machinery. It has previously been shown that Nsp1 from SARS-CoV inhibits the host response to viral RNA by inhibiting host cell translation including mRNA encoding Type 1 interferon (3). The ability of SARS-CoV-2 Nsp1 has also been shown to exert inhibition of host cell translation, specifically by binding to the 40S ribosomal subunit, and competing out entry of M⁷G capped eukaryotic mRNA to the entry channel of the pre-initiation translation complex (4,5).