SARS-CoV-2 Membrane Protein (E3V5H) Mouse mAb #32904
Filter:
- WB
Supporting Data
| REACTIVITY | Vir |
| SENSITIVITY | Endogenous |
| MW (kDa) | 20-30 |
| Source/Isotype | Mouse IgG3 |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- Vir-Virus
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
SARS-CoV-2 Membrane Protein (E3V5H) Mouse mAb recognizes endogenous levels of total SARS-CoV-2 membrane protein in infected cells. In some transfected models, a band of unknown origin, but not virus related, may be observed at approximately 50 kDa.
Species Reactivity:
Virus
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala183 of SARS-CoV-2 membrane protein.
Background
The cause of the COVID-19 pandemic is a novel and highly pathogenic coronavirus, termed SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2). SARS-CoV-2 is a member of the Coronaviridae family of viruses (1). The genome of SARS-CoV-2 is relatively large and encodes up to 29 open reading frames (ORFs). These include ORF1a and ORF1b (further processed into 16 non-structural proteins), 9 accessory proteins, and 4 canonical structural proteins: spike (S), envelope (E), membrane (M), and nucleocapsid (N) (2). Membrane protein is transcribed and translated from nested subgenomic RNA of the SARS-CoV-2 genome. Interaction of the membrane protein with the viral nucleocapsid protein is thought to stabilize the RNA-protein complex in the virions (3). Membrane protein has also been suggested to interact with the viral spike protein, but the functional consequence of that interaction is currently unclear (4).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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