R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
RTN3 (F5X9D) Rabbit mAb #59460
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 25 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
RTN3 (F5X9D) Rabbit mAb recognizes endogenous levels of total RTN3 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human RTN3 protein.
Background
Reticulon-3 (RTN3), a member of the reticulon protein family, plays a crucial role in maintaining the tubular shape and structural integrity of the endoplasmic reticulum (ER) network (1,2). The longest isoform of RTN3 (Rtn3L) carries six active LC3-interacting region domains, which are essential for LC3/GABARAP binding, fragmentation of ER tubules, and its function as an ER-phagy receptor (3,4). Rtn3L is also recruited to ER-endosome membrane contact sites by the endosomal protein Rab9a, where it promotes endosome maturation and cargo sorting (5). RTN3 is highly expressed in neuronal tissues and is a negative modulator of β-amyloid converting enzyme 1 (BACE1), the enzyme that cleaves amyloid precursor protein (APP) to release β-amyloid peptides (6). Therefore, altered expression of RTN3 may modulate amyloid plaque formation, a hallmark of Alzheimer's disease (7,8).
- Voeltz, G.K. et al. (2006) Cell 124, 573-86.
- Chen, Y.J. et al. (2020) J Cell Biol 219, e201908182. doi: 10.1083/jcb.201908182.
- Grumati, P. et al. (2017) Elife 6, doi: 10.7554/eLife.25555.
- Yang, M. et al. (2021) Front Cell Dev Biol 9, 684526.
- Wu, H. and Voeltz, G.K. (2021) Dev Cell 56, 52-66.e7.
- He, W. et al. (2004) Nat Med 10, 959-65.
- Shi, Q. et al. (2009) J Neurosci 29, 9163-73.
- Sharoar, M.G. and Yan, R. (2017) Rev Neurosci 28, 145-154.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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