R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
RPRD1B (E8J9U) Rabbit mAb #50156
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 37 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
RPRD1B (E8J9U) Rabbit mAb recognizes endogenous levels of total RPRD1B protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human RPRD1B protein.
Background
Regulation of nuclear pre-mRNA domain-containing 1B (RPRD1B), also known as cell cycle-related and expression-elevated protein in tumor (CREPT), is a transcriptional regulator that interacts with the POLR2A catalytic subunit of RNA polymerase II (Pol II) (1). It is also an oncoprotein that promotes cell proliferation and is upregulated in a variety of cancers, including gastric, colorectal, and non-small cell lung cancer (NSCLC) (2-4). RPRD1B contains a CTD-interacting domain (CID), which interacts with the C-terminal domain (CTD) of Pol II to regulate transcription termination (5). It is believed that this association enhances the binding of Pol II to the CCND1 promoter, thereby increasing cyclin D1 transcription and promoting tumorigenesis (6). Specifically, RPRD1B promotes chromatin looping of the CCND1 gene to recycle Pol II from the termination site to the promoter region (6). RPRD1B may also regulate the transcription of various genes through associations with STAT3 and the histone acetyltransferase p300 (7,8).
- Ni, Z. et al. Transcription 2, 237-42.
- Ding, L. et al. (2018) Cell Death Dis 9, 1172.
- Zhang, Y. et al. (2018) Oncogene 37, 3485-3500.
- Jung, H.M. et al. (2009) J Cell Biochem 106, 703-13.
- Ali, I. et al. (2019) Mol Cell 74, 1164-1174.e4.
- Lu, D. et al. (2012) Cancer Cell 21, 92-104.
- Zhang, Y. et al. (2014) J Biol Chem 289, 22589-22599.
- Zhai, W. et al. (2021) Br J Cancer 124, 1437-1448.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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