R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
RPL15 (E3X8M) Rabbit mAb #29753
Filter:
- WB
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 24 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
RPL15 (E3X8M) Rabbit mAb recognizes endogenous levels of total RPL15 protein. This antibody detects a nonspecific signal around 34 kDa in lysates of some cell lines.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human RPL15 protein.
Background
Ribosomal protein L15 (RPL15) is a structural component of the large 60S subunit of the ribosome. Studies show that overexpressed RPL15 protein promotes metastatic growth of breast cancer patient-derived circulating tumor cells. Overexpression of RPL15 was shown to increase translation of other ribosomal proteins of large and small subunits and cell cycle regulators (1). Furthermore, the DNA topoisomerase I inhibitor topotecan binds to RPL15 and suppresses preribosomal subunit formation, which causes ribosomal stress and enhances DNA topoisomerase I-independent antitumor immune activation mediated by damage-associated molecular patterns (2). In addition, RPL15 expression is upregulated in colon cancer cells. RPL15 deficiency leads to ribosomal stress and, therefore, apoptosis of colon cancer cells (3).
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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