R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
ROCK2 (E5T5P) Rabbit mAb #47012
Filter:
- WB
- IP
- IF
Western blot analysis of extracts from various cell lines using ROCK2 (E5T5P) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 160 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Related Products
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:10 - 1:50 |
| Immunoprecipitation | 1:200 |
| Immunofluorescence (Immunocytochemistry) | 1:200 - 1:800 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
ROCK2 (E5T5P) Rabbit mAb recognizes endogenous levels of total ROCK2 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala1383 of human ROCK2 protein.
Background
ROCK (Rho-associated kinase), a family of serine/threonine kinases, is an important downstream target of Rho-GTPase and plays an important role in Rho-mediated signaling. Two isoforms of ROCK have been identified: ROCK1 and ROCK2. ROCK is composed of N-terminal catalytic, coiled-coil, and C-terminal PH (pleckstrin homology) domains. The C-terminus of ROCK negatively regulates its kinase activity (1,2). ROCK1 is cleaved by caspase-3 at a conserved DETD1113/G sequence resulting in loss of its C-terminal inhibitory domain (3). ROCK2 is directly cleaved by granzyme B (grB). Cleavage activates ROCK and leads to phosphorylation of myosin light chain (MLC) and inhibition of myosin phosphatase (4). This phosphorylation may account for the mechanism by which Rho regulates cytokinesis, cell motility, cell membrane blebbing during apoptosis, and smooth muscle contraction (5-7).
- Nakagawa, O. et al. (1996) FEBS Lett. 392, 189-193.
- Lee, J.H. et al. (2004) J. Cell. Biol. 167, 327-337.
- Sebbagh, M. et al. (2001) Nat Cell Biol 3, 346-52.
- Sebbagh, M. et al. (2005) J Exp Med 201, 465-71.
- Amano, M. et al. (1996) J Biol Chem 271, 20246-9.
- Kureishi, Y. et al. (1997) J Biol Chem 272, 12257-60.
- Totsukawa, G. et al. (2000) J Cell Biol 150, 797-806.
Pathways
Explore pathways related to this product.
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