RNMT Antibody #57407
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 60 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
RNMT Antibody recognizes endogenous levels of total RNMT protein.
Species Reactivity:
Human, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly40 of human RNMT protein. Antibodies are purified by peptide affinity chromatography.
Background
RNA guanine-7 methyltransferase (RNMT) is the enzyme responsible for providing the methyl group on the 7 position on the guanosine at the 5’ cap of mRNAs. The methyl group provides a docking site for eIF4E and subsequent formation of the eIF4F complex to promote translation (1-4). RNMT binds along with activating subunit RNMT-activating miniprotein (RAM), which is required for efficient methylation of the mRNA cap (5). In addition to the methyltransferase activity, RNMT-RAM has been shown to affect RNA pol II occupancy, and knockdown has been shown to reduce mRNA production (6,7). RNMT-RAM has also been shown to play a role in rRNA synthesis by controlling RNA pol I activity through controlling expression of c-Myc (8).
- Pillutla, R.C. et al. (1998) Genomics 54, 351-3.
- Yokoska, J. et al. (2000) Biochem Biophys Res Commun 268, 617-24.
- Furuichi, Y. and Shatkin, A.J. (2000) Adv Virus Res 55, 135-84.
- Gingras, A.C. et al. (1999) Annu Rev Biochem 68, 913-63.
- Gonatopoulos-Pournatzis, T. et al. (2011) Mol Cell 44, 585-96.
- Varshney, D. et al. (2018) Cell Rep 23, 1530-1542.
- Cowling, V.H. and Cole, M.D. (2010) Genes Cancer 1, 576-579.
- Dunn, S. et al. (2017) Biochem J 474, 377-384.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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