渲染靶标:SSR
Render Timestamp: 2025-03-14T04:38:19.433Z
Commit: 9fc0f116116d9da247dc8ddd4e5fe811153412e1
XML generation date: 2024-09-30 01:57:27.449
Product last modified at: 2025-01-01T09:04:11.952Z
1% for the Planet 标识
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

RhoG (E9B7Z) Rabbit mAb #60370

Filter:
  • WB
Western Blotting Image 1: RhoG (E9B7Z) Rabbit mAb
Western blot analysis of extracts from various cell lines using RhoG (E9B7Z) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

To Purchase # 60370

Supporting Data

REACTIVITY H M R
SENSITIVITY Endogenous
MW (kDa) 20
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

RhoG (E9B7Z) Rabbit mAb recognizes endogenous levels of total RhoG protein. This antibody does not cross-react with Rac1 or Cdc42 proteins.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala144 of human RhoG protein.

Background

Rho family small GTPases, including Rho, Rac and Cdc42, act as molecular switches, regulating processes such as cell migration, adhesion, proliferation, and differentiation. They are activated by guanine nucleotide exchange factors (GEFs), which catalyze the exchange of bound GDP for GTP, and they are inhibited by GTPase activating proteins (GAPs), which catalyze the hydrolysis of GTP to GDP. A third level of regulation is provided by the stoichiometric binding of Rho GDP dissociation inhibitor (RhoGDI) (1).
RhoG, a Rho family GTPase similar to Rac1 and Cdc42, regulates actin dynamics and cell migration.
RhoG regulates the microtubule-mediated formation of focal adhesions (2), as well as tube formation in vascular endothelial cells (3). RhoG mediates tumor cell invasion in glioblastoma, and regulates paxillin phosphorylation and invadopodia formation in breast cancer cells (4,5).

Pathways

Explore pathways related to this product.


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit our Trademark Information page.