R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
RhoG (E9B7Z) Rabbit mAb #60370
Filter:
- WB
Western blot analysis of extracts from various cell lines using RhoG (E9B7Z) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 20 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Related Products
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
RhoG (E9B7Z) Rabbit mAb recognizes endogenous levels of total RhoG protein. This antibody does not cross-react with Rac1 or Cdc42 proteins.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala144 of human RhoG protein.
Background
Rho family small GTPases, including Rho, Rac and Cdc42, act as molecular switches, regulating processes such as cell migration, adhesion, proliferation, and differentiation. They are activated by guanine nucleotide exchange factors (GEFs), which catalyze the exchange of bound GDP for GTP, and they are inhibited by GTPase activating proteins (GAPs), which catalyze the hydrolysis of GTP to GDP. A third level of regulation is provided by the stoichiometric binding of Rho GDP dissociation inhibitor (RhoGDI) (1).
RhoG, a Rho family GTPase similar to Rac1 and Cdc42, regulates actin dynamics and cell migration.
RhoG regulates the microtubule-mediated formation of focal adhesions (2), as well as tube formation in vascular endothelial cells (3). RhoG mediates tumor cell invasion in glioblastoma, and regulates paxillin phosphorylation and invadopodia formation in breast cancer cells (4,5).
RhoG, a Rho family GTPase similar to Rac1 and Cdc42, regulates actin dynamics and cell migration.
RhoG regulates the microtubule-mediated formation of focal adhesions (2), as well as tube formation in vascular endothelial cells (3). RhoG mediates tumor cell invasion in glioblastoma, and regulates paxillin phosphorylation and invadopodia formation in breast cancer cells (4,5).
Pathways
Explore pathways related to this product.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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