R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
RhoA (67B9) Rabbit mAb #2117
Filter:
- WB
Supporting Data
| REACTIVITY | H M R Mk B |
| SENSITIVITY | Endogenous |
| MW (kDa) | 21 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
- B-Bovine
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:50 - 1:250 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
RhoA (67B9) Rabbit mAb recognizes endogenous levels of total RhoA protein. The antibody does not recognize the related proteins RhoB or RhoC.
Species Reactivity:
Human, Mouse, Rat, Monkey, Bovine
Source / Purification
Monoclonal antibody is produced by immunizing animals with synthetic peptides corresponding to residues near the carboxy terminus of human RhoA.
Background
Rho family small GTPases, including Rho, Rac and cdc42, act as molecular switches, regulating processes such as cell migration, adhesion, proliferation and differentiation. They are activated by guanine nucleotide exchange factors (GEFs), which catalyze the exchange of bound GDP for GTP, and inhibited by GTPase activating proteins (GAPs), which catalyze the hydrolysis of GTP to GDP. A third level of regulation is provided by the stoichiometric binding of Rho GDP dissociation inhibitor (RhoGDI) (1). RhoA, RhoB and RhoC are highly homologous, but appear to have divergent biological functions. Carboxy-terminal modifications and differences in subcellular localization allow these three proteins to respond to and act on distinct signaling molecules (2,3).
Functions of RhoA, the most highly studied of these three, include regulation of actomyosin contractility (4), cytokinesis (5), focal adhesion assembly (6) and cell polarity (7).
Functions of RhoA, the most highly studied of these three, include regulation of actomyosin contractility (4), cytokinesis (5), focal adhesion assembly (6) and cell polarity (7).
- DerMardirossian, C. and Bokoch, G.M. (2005) Trends Cell Biol 15, 356-63.
- Wennerberg, K. and Der, C.J. (2004) J Cell Sci 117, 1301-12.
- Wheeler, A.P. and Ridley, A.J. (2004) Exp Cell Res 301, 43-9.
- Bi, D. et al. (2005) Circ Res 96, 890-7.
- Kimura, K. et al. (2000) J Biol Chem 275, 17233-6.
- Barry, S.T. and Critchley, D.R. (1994) J Cell Sci 107 ( Pt 7), 2033-45.
- Van Keymeulen, A. et al. (2006) J Cell Biol 174, 437-45.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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