RGS4 Antibody #9195
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Inquiry Info. # 9195
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Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 23 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
RGS4 Antibody recognizes endogenous levels of total RGS4 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Cys33 of human RGS4 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Regulators of G protein signaling (RGS) are a family of about 40 proteins that determine the signaling amplitude and duration of G protein-coupled receptor signaling via modulation of the GTPase activity of G proteins (1-3). Each RGS has a distinct structure, expression pattern, and regulation, resulting in preferential interactions with receptors, G proteins, and other signaling proteins, as well as a unique function (4).
RGS4 is a small RGS of 23 kDa that belongs to the R4 subfamily of RGS. It harbors a short N-terminus thought to be critical for its functional specificity towards GPCRs and a RGS homology domain (RH domain) (5). RGS4 shares activity at both Gαq (6) and Gαi/o proteins (7).
RGS4 degradation is regulated by opioid receptor-mediated ubiquitination after stimulation by opioid receptor agonists such as DAMGO (8); and RGS4 modulates the action of morphine and other opiates in vivo (9).
RGS4 is a small RGS of 23 kDa that belongs to the R4 subfamily of RGS. It harbors a short N-terminus thought to be critical for its functional specificity towards GPCRs and a RGS homology domain (RH domain) (5). RGS4 shares activity at both Gαq (6) and Gαi/o proteins (7).
RGS4 degradation is regulated by opioid receptor-mediated ubiquitination after stimulation by opioid receptor agonists such as DAMGO (8); and RGS4 modulates the action of morphine and other opiates in vivo (9).
- Hollinger, S. and Hepler, J.R. (2002) Pharmacol Rev 54, 527-59.
- Traynor, J.R. and Neubig, R.R. (2005) Mol Interv 5, 30-41.
- Neubig, R.R. and Siderovski, D.P. (2002) Nat Rev Drug Discov 1, 187-97.
- Terzi, D. et al. (2009) Prog Mol Biol Transl Sci 86, 299-333.
- Hollinger, S. and Hepler, J.R. (2002) Pharmacol Rev 54, 527-59.
- Xu, X. et al. (1999) J Biol Chem 274, 3549-56.
- Berman, D.M. et al. (1996) Cell 86, 445-52.
- Wang, Q. and Traynor, J.R. (2011) J Biol Chem 286, 7854-64.
- Han, M.H. et al. (2010) Biol Psychiatry 67, 761-9.
限制使用
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