R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
RGS14 (E4N2Q) Rabbit mAb #76703
Filter:
- WB
- IF
Supporting Data
| REACTIVITY | M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 65 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Frozen) | 1:3200 - 1:6400 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #74575.
For a carrier free (BSA and azide free) version of this product see product #74575.
Protocol
Specificity / Sensitivity
RGS14 (E4N2Q) Rabbit mAb recognizes endogenous levels of total RGS14 protein. Non-specific staining in fixed frozen mouse kidney may be observed by immunofluorescence.
Species Reactivity:
Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse RGS14 protein.
Background
Regulator of G-protein signaling (RGS) proteins are a structurally diverse family of more than 30 member proteins enriched in brain that typically limit neurotransmitter receptor- and G protein-linked signaling in the CNS and elsewhere by serving as GTPase-activating proteins (GAPs) (1). RGS14 is a multifunctional signaling protein that regulates post-synaptic plasticity in neurons and acts as a scaffolding protein that integrates G protein, Ras/ERK, and calcium/calmodulin signaling pathways essential for spine plasticity and cell signaling (2). It is highly enriched within pyramidal cells of hippocampal area CA2, allowing RGS14 to naturally suppress long-term potentiation (LTP) and structural plasticity in that region (2-4). RGS14 has been shown to directly interact with two key Ca2+-activated proteins in CA2 neurons: calcium/calmodulin and CaMKII (5).
- Lee, S.E. et al. (2010) Proc Natl Acad Sci USA 107, 16994-8.
- Harbin, N.H. et al. (2021) Int J Mol Sci 22, 6823. doi: 10.3390/ijms22136823.
- Harbin, N.H. et al. (2023) Neurobiol Dis 181, 106128.
- Evans, P.R. et al. (2018) eNeuro 5, ENEURO.0353-17.2018. doi: 10.1523/ENEURO.0353-17.2018.
- Evans, P.R. et al. (2018) J Proteome Res 17, 1700-1711.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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