R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
REST (E3L2I) Rabbit mAb #88188
Filter:
- WB
- ChIP
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 200 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- ChIP-Chromatin Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
For optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Chromatin IP | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
REST (E3L2I) Rabbit mAb recognizes endogenous levels of total REST protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human REST protein.
Background
Repressor element 1-silencing transcription factor (REST), also known as neural-restrictive silencer factor (NRSF), is a repressive transcription factor that acts by binding to neuron-restrictive silencer elements present in many neuronal genes (1,2). The C-terminal domain of REST is bound by its cofactor, CoREST, which in turn recruits many epigenetic regulators, including HDAC1/2, Sin3A, MeCP2, and LSD1 (3-5). REST is capable of local and long-range chromatin remodeling, including changes to nucleosome phasing and histone modifications (6,7). REST also plays a key role in miRNA regulation along with CREB through the control of genes involved in miRNA biogenesis (8). Many of the miRNAs regulated by REST exist in feedback loops, which control neural stem cell self-renewal and maintenance (9,10). REST also is capable of regulating many long non-coding RNAs (11). REST and its regulatory network of genes and RNAs have been implicated in numerous neurological disorders, including schizophrenia, Huntington’s, and Alzheimer’s disease (12-14).
- Schoenherr, C.J. and Anderson, D.J. (1995) Science 267, 1360-3.
- Kabell, G. et al. (1996) Am J Cardiol 78, 1443-6.
- Andrés, M.E. et al. (1999) Proc Natl Acad Sci USA 96, 9873-8.
- Qureshi, I.A. et al. (2010) Cell Cycle 9, 4477-86.
- Huang, Y. et al. (1999) Nat Neurosci 2, 867-72.
- Zheng, D. et al. (2009) Genome Biol 10, R9.
- Lunyak, V.V. et al. (2002) Science 298, 1747-52.
- Wu, J. and Xie, X. (2006) Genome Biol 7, R85.
- Conaco, C. et al. (2006) Proc Natl Acad Sci USA 103, 2422-7.
- Laneve, P. et al. (2010) Nucleic Acids Res 38, 6895-905.
- Johnson, R. et al. (2009) RNA 15, 85-96.
- Alsaqati, M. et al. (2022) Transl Psychiatry 12, 438.
- Conforti, P. et al. (2013) J Neurochem 127, 22-35.
- Lu, T. et al. (2014) Nature 507, 448-54.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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