R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
RBM4 (E1Y2G) Rabbit mAb #61689
Filter:
- WB
- IP
- IF
Supporting Data
| REACTIVITY | H Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 41 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunofluorescence (Immunocytochemistry) | 1:50 - 1:200 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
RBM4 recognizes endogenous levels of total RBM4 protein. This antibody does not cross-react with RBM4B protein.
Species Reactivity:
Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro317 of human RBM4 protein.
Background
RBM4 functions as an RNA-binding protein and precursor mRNA splicing factor that antagonizes activity of PTBP1-mediated splicing (1-4). Upon phosphorylation at Ser309, RBM4 accumulates in stress granules and suppresses cap-dependent translation through eIF4A (3). Exon skipping of MEF2C by RBM4 has been shown to play a key role in the differentiation of brown adipocytes (5). The splicing activities of RBM4 seem to suppress tumorigenesis in the context of multiple cancer types (5-8).
- Lai, M.C. et al. (2003) EMBO J 22, 1359-69.
- Lin, J.C. and Tarn, W.Y. (2005) Mol Cell Biol 25, 10111-21.
- Lin, J.C. et al. (2007) Proc Natl Acad Sci USA 104, 2235-40.
- Lin, J.C. and Tarn, W.Y. (2011) J Cell Biol 193, 509-20.
- Chen, J.Y. et al. (2017) Onco Targets Ther 10, 339-45.
- Lin, J.C. et al. (2017) Sci Rep 7, 44204.
- Lin, J.C. et al. (2018) Biochim Biophys Acta Mol Cell Res 1865, 259-72.
- Yong, H. et al. (2019) Med Sci Monit 25, 4025-34.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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