RBM15 Antibody #25261
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 110 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
RBM15 Antibody recognizes endogenous levels of total RBM15 protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala557 of human RBM15 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
RBM15 is an RNA-binding protein that is part of the WTAP-METTL3 m6A methyltransferase complex. RBM15 and related RBM15B interact with WTAP to recruit the complex to target mRNAs, and are critical to XIST-mediated gene silencing (1). RBM15 can recruit splicing factors such as SF3B1 to mRNA to promote alternative splicing. Expression levels of RBM15 can be regulated by PRMT1, which can methylate R578, resulting in RBM15 ubiquitinylation and degradation (2). This process is critical in acute megakaryoblastic leukemia, a cancer type where RBM15 is fused to the MKL-1 gene and PRMT1 is overexpressed (3). RBM15 normally plays roles in hematopoietic development and myeloid differentiation, where it can regulate the levels of c-Myc (4-6).
- Patil, D.P. et al. (2016) Nature 537, 369-73.
- Zhang, L. et al. (2015) Elife 4, e07938. doi: 10.7554/eLife.07938.
- Ma, Z. et al. (2001) Nat Genet 28, 220-1.
- Ma, X. et al. (2007) Mol Cell Biol 27, 3056-64.
- Raffel, G.D. et al. (2007) Proc Natl Acad Sci U S A 104, 6001-6.
- Niu, C. et al. (2009) Blood 114, 2087-96.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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