R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
RBM10 (E5V5K) Rabbit mAb #47729
Filter:
- WB
- IP
- eCLIP
Supporting Data
| REACTIVITY | H Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 110, 135 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- eCLIP-eCLIP
Species Cross-Reactivity Key:
- H-Human
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| eCLIP | 1:200 |
For more information about the RBP-eCLIP service please visit Eclipsebio.
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
RBM10 (E5V5K) Rabbit mAb recognizes endogenous levels of total RBM10 protein.
Species Reactivity:
Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His919 of human RBM10 protein.
Background
RBM10 is an RNA binding protein and a paralog to tumor suppressor RBM5 (1,2). Alternative splicing creates two highly expressed variants that differ by one exon. RBM10 controls alternative splicing and exon skipping of mRNAs, including Fas and Bcl-x (3,4). RBM10 has been shown to affect apoptosis via up regulation of TNF-α mRNA (5). In lung cancer, RBM10 mutations disrupt the splicing of NUMB, a regulator of Notch signaling (6,7). Fusion of the RBM10 gene with TFE3 has been found in renal cell carcinoma (8-9). Mutations of RBM10 causes TARP syndrome, an x-linked, lethal disorder characterized by various developmental defects (10-12).
- Timmer, T. et al. (1999) Genomics 60, 238-40.
- Bonnal, S. et al. (2008) Mol Cell 32, 81-95.
- Wang, Y. et al. (2013) EMBO Mol Med 5, 1431-42.
- Inoue, A. et al. (2014) FEBS Lett 588, 942-7.
- Wang, K. et al. (2012) J Cell Death 5, 1-19.
- Bechara, E.G. et al. (2013) Mol Cell 52, 720-33.
- Hernández, J. et al. (2016) RNA Biol 13, 466-72.
- Just, P.A. et al. (2016) Genes Chromosomes Cancer 55, 541-8.
- Argani, P. et al. (2017) Am J Surg Pathol 41, 655-62.
- Gorlin, R.J. et al. (1970) Am J Dis Child 119, 176-8.
- Johnston, J.J. et al. (2010) Am J Hum Genet 86, 743-8.
- Gripp, K.W. et al. (2011) Am J Med Genet A 155A, 2516-20.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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