RalA Antibody #3526
Filter:
- WB
Supporting Data
| REACTIVITY | H Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 25 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
RalA Antibody detects endogenous levels of total human RalA protein.
Species Reactivity:
Human, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala142 of human RalA. Antibodies are purified by peptide affinity chromatography.
Background
RalA and RalB are members of the Ras family of small GTPases and are highly homologous in protein sequence. The functions of RalA and RalB are distinct yet overlapping. By binding to various effector proteins, RalA and RalB serve as important GTP sensors for exocytosis and membrane trafficking (1-3). RalA is required for Ras-related tumorigenesis (4) and RalB is important for tumor survival (5). In addition to tumor formation, Ral proteins also play a role in cancer cell migration and metastatic tumor invasion (6,7).
- Li, G. et al. (2007) J Neurosci 27, 190-202.
- van Dam, E.M. and Robinson, P.J. (2006) Int J Biochem Cell Biol 38, 1841-7.
- Camonis, J.H. and White, M.A. (2005) Trends Cell Biol 15, 327-32.
- Lim, K.H. et al. (2005) Cancer Cell 7, 533-45.
- Chien, Y. et al. (2006) Cell 127, 157-70.
- Oxford, G. et al. (2005) Cancer Res 65, 7111-20.
- Lim, K.H. et al. (2006) Curr Biol 16, 2385-94.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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