R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Rabex-5 (D21F12) Rabbit mAb #7622
Filter:
- WB
- IP
Western blot analysis of extracts from SH-SY5Y cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® Rabex-5 siRNA I (+), using Rabex-5 (D21F12) Rabbit mAb #7622 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The Rabex-5 (D21F12) Rabbit mAb confirms silencing of Rabex-5 expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 60-80 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Related Products
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Rabex-5 (D21F12) Rabbit mAb recognizes endogenous levels of total Rabex-5 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Rabex-5 protein.
Background
Rabex-5, also called RabGEF1 and RAP1, was identified as a guanine nucleotide exchange factor (GEF) for Rab5, a member of the Ras superfamily of small Rab GTPases (1). Rabex-5 generates the GTP-bound active form of Rab5 and forms a tight association with its effector protein Rabaptin-5 (2). This complex localizes to endosomal membranes where it functions as a key regulator of vesicular trafficking during early endocytosis (3,4). Rabex-5 is also monoubiquitinated and has ubiquitin ligase activity that regulates its recruitment to early endosomes (5,6). The conformational change between Rab5 GTP/GDP states is essential for its biological function as a rate limiting regulator at multiple steps during endocytosis (5). Through its control of endosomal trafficking and endocytosis, Rabex-5 has been shown to negatively regulate NGF-mediated neurite outgrowth (7) as well as FcεRI-dependent mast cell activation (8).
- Horiuchi, H. et al. (1997) Cell 90, 1149-59.
- Lippé, R. et al. (2001) Mol Biol Cell 12, 2219-28.
- Zerial, M. and McBride, H. (2001) Nat Rev Mol Cell Biol 2, 107-17.
- van der Bliek, A.M. (2005) Nat Cell Biol 7, 548-50.
- Mattera, R. et al. (2006) J Biol Chem 281, 6874-83.
- Mattera, R. and Bonifacino, J.S. (2008) EMBO J 27, 2484-94.
- Liu, J. et al. (2007) Mol Biol Cell 18, 1375-84.
- Tam, S.Y. et al. (2004) Nat Immunol 5, 844-52.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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