R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Rab27B (E4V3O) Rabbit mAb #17572
Filter:
- WB
- IP
- IHC
- IF
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 27 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunohistochemistry (Paraffin) | 1:200 - 1:800 |
| Immunofluorescence (Immunocytochemistry) | 1:800 - 1:3200 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Rab27B (E4V3O) Rabbit mAb recognizes endogenous levels of total Rab27B protein. This antibody does not cross-react with Rab27A protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly208 of human Rab27B protein.
Background
Rab27 proteins are members of the Ras superfamily of small Rab GTPases associated with the process of exocytosis (1-2), and in particular the exosome secretion pathway (3-5). There are two Rab27 isoforms, Rab27A and Rab27B, which are encoded by separate genes and which exhibit distinct subcellular localization and functions. Rab27A colocalizes to CD63+ vesicles, whereas Rab27B is observed in perinuclear vesicles. Targeted knockdown studies suggest that the isoforms control distinct steps of the exosome secretion pathway (6). Rab27A participates in docking and membrane fusion from multivesicular endosomes (MVEs) to exosomes, whereas Rab27B mediates the transfer of membrane vesicles from the trans-Golgi network to MVEs. It has been suggested that Rab27A and Rab27B may have importance in exosome secretion by cancer cells (7).
- Fukuda, M. (2013) Traffic 14, 949-63.
- Izumi, T. (2007) Endocr J 54, 649-57.
- Elstak, E.D. et al. (2011) Blood 118, 1570-8.
- Bahadoran, P. et al. (2001) J Cell Biol 152, 843-50.
- Wood, S.M. et al. (2009) Blood 114, 4117-27.
- Ostrowski, M. et al. (2010) Nat Cell Biol 12, 19-30; sup pp 1-13.
- Li, Z. et al. (2018) Cell Commun Signal 16, 44.
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