R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Rab27A (D7Z9Q) Rabbit mAb #69295
Filter:
- WB
- IP
- IF
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 27 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunofluorescence (Immunocytochemistry) | 1:800 - 1:1600 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Rab27A (D7Z9Q) Rabbit mAb recognizes endogenous levels of total Rab27a protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Rab27a protein.
Background
Rab27 is a member of the Ras superfamily of small Rab GTPases implicated in exocytosis (1-2). The protein is localized in secretory lysosomes, such as melanosomes in melanocyte or lytic granules in cytotoxic T cells to control exosome secretion pathway (3-5). Rab27 has two isoforms, Rab27a and Rab27b. Rab27a colocalizes with part of CD63 staining vesicles, and Rab27b shows perinuclear distribution. Target knock out studies indicate that the isoforms control different steps of the exosome secretion pathway (6). Rab27a interacts with a wide range of effectors and is involved in multiple steps of exocytosis depending on the effector it associated with and the cell type that is involved (1,2). Rab27a has been shown to be an important player in leukocyte function, cancer metastasis and invasion, and insulin secretion (7-11)
- Fukuda, M. (2013) Traffic 14, 949-63.
- Izumi, T. (2007) Endocr J 54, 649-57.
- Elstak, E.D. et al. (2011) Blood 118, 1570-8.
- Bahadoran, P. et al. (2001) J Cell Biol 152, 843-50.
- Wood, S.M. et al. (2009) Blood 114, 4117-27.
- Ostrowski, M. et al. (2010) Nat Cell Biol 12, 19-30; sup pp 1-13.
- Catz, S.D. (2013) J Leukoc Biol 94, 613-22.
- Herrero-Turrión, M.J. et al. (2008) J Immunol 181, 3793-803.
- Ostenfeld, M.S. et al. (2014) Cancer Res 74, 5758-71.
- Dong, W. et al. (2015) Discov Med 20, 357-67.
- Cazares, V.A. et al. (2014) Traffic 15, 997-1015.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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