R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Rab1A (D5F8M) Rabbit mAb #13099
Filter:
- WB
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 22 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Rab1A (D5F8M) Rabbit mAb recognizes endogenous levels of total Rab1A protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys103 of human Rab1A protein.
Background
Ras-related protein Rab1A (Rab1A) is a member of the Ras superfamily of cellular G proteins that function in protein transport and membrane restructuring (1). Early immunofluorescence studies determined that Rab1A localizes to a region between the endoplasmic reticulum (ER) and the Golgi complex, and in early Golgi compartments (2). Rab1A binds and recruits the COPII complex tethering factor p115 to a cis-SNARE complex associated with COPII-coated, budding vesicles on the endoplasmic reticulum (3). A Rab1 effector complex containing several proteins, including the cis-Golgi tethering protein GM130 and the stacking protein GRASP65, is essential for targeting and fusion of COPII-coated vesicles with the Golgi complex (4). Rab1A also interacts with the golgin tethering and docking proteins giantin (GOLGB1) and golgin-84 to regulate Golgi structure formation and function (5,6). Thus, Rab1A plays an important role in mediating the export of newly synthesized target proteins from ER to the Golgi. As with other Rab proteins, Rab1A function requires an intrinsic GTPase cycling activity facilitated by associated GEF and GAP factors (7-9). In addition to mediating ER to Golgi transport, Rab1A is also involved in autophagy during early autophagosome formation (10,11).
- Zerial, M. and McBride, H. (2001) Nat Rev Mol Cell Biol 2, 107-17.
- Saraste, J. et al. (1995) J Cell Sci 108 ( Pt 4), 1541-52.
- Allan, B.B. et al. (2000) Science 289, 444-8.
- Moyer, B.D. et al. (2001) Traffic 2, 268-76.
- Koreishi, M. et al. (2013) PLoS One 8, e59821.
- Satoh, A. et al. (2003) Traffic 4, 153-61.
- Nuoffer, C. et al. (1994) J Cell Biol 125, 225-37.
- Pind, S.N. et al. (1994) J Cell Biol 125, 239-52.
- Haas, A.K. et al. (2007) J Cell Sci 120, 2997-3010.
- Huang, J. et al. (2011) Autophagy 7, 17-26.
- Lipatova, Z. et al. (2013) Mol Biol Cell , .
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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