Rab11b Antibody #2414
Filter:
- WB
Supporting Data
| REACTIVITY | H M R B |
| SENSITIVITY | Endogenous |
| MW (kDa) | 25 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- B-Bovine
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Rab11b Antibody detects endogenous levels of total Rab11b protein.
Species Reactivity:
Human, Mouse, Rat, Bovine
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding residue surrounding Arg184 of human Rab11b protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Rab11a, Rab11b and Rab25 are members of the Rab11 family of small Ras-like GTPases. Rab11 (isoforms Rab11a and Rab11b) functions as a key regulator in the recycling of perinuclear, plasma membrane and Golgi compartment endosomes (1,2). Despite some overlap, distinct differences exist between Rab11a and Rab11b in both their cellular distribution and functional roles. Rab11a is ubiquitously expressed while Rab11b is found mainly in the heart and brain (3,4). Like other Rab proteins, Rab11 exerts its function via interactions with Rab11 family interacting proteins (FIPs). While there are three distinct classes of FIPs, all appear to share a conserved carboxy-terminal Rab-binding domain that allows Rab-FIP protein interaction. When bound together, these proteins are thought to regulate membrane-associated protein sorting (5,6).
- Ullrich, O. et al. (1996) J. Cell Biol. 135, 913-924.
- Chen, W. et al. (1998) Mol. Biol. Cell 9, 3241-3257.
- Lapierre, L.A. et al. (2003) Exp. Cell Res. 290, 322-331.
- Khvotchev, M.V. et al. (2003) J. Neurosci. 23, 10531-10539.
- Junutula, J.R. et al. (2004) J. Biol. Chem. 279, 33430-33437.
- Hales, C.M. et al. (2001) J. Biol. Chem. 276, 39067-39075.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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