R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Pyk2 (F3I2K) Rabbit mAb #43550
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 116 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:10 - 1:50 |
| Immunoprecipitation | 1:100 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Pyk2 (F3I2K) Rabbit mAb recognizes endogenous levels of total Pyk2 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the amino terminus of human Pyk2 protein.
Background
Protein-tyrosine kinase 2 (Pyk2), also called CAKβ, RAFTK, and CADTK, is a nonreceptor tyrosine kinase structurally related to focal adhesion kinase (FAK) (1-4). Pyk2 is predominantly expressed in cells derived from hematopoietic lineages and in the central nervous system. Pyk2 is one of the signaling mediators for the G protein-coupled receptors and MAP kinase signaling pathway. It plays an important role in cell spreading and migration (5-7).
- Avraham, S. et al. (1995) J. Biol. Chem. 270, 27742-27751.
- Lev, S. et al. (1995) Nature 376, 737-745.
- Sasaki, H. et al. (1995) J. Biol. Chem. 270, 21206-21219.
- Yu, H. et al. (1996) J. Biol. Chem. 271, 29993-29998.
- Duong, L.T. et al. (2001) J Biol Chem 276, 7484-92.
- Watson, J.M. et al. (2001) J Biol Chem 276, 3536-42.
- Tang, H. et al. (2002) J Biol Chem 277, 5441-7.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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