R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
PTP-PEST (D4W7W) Rabbit mAb #14735
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 110 to 125 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
PTP-PEST (D4W7W) Rabbit mAb recognizes endogenous levels of total PTP-PEST protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg747 of human PTP-PEST protein.
Background
PTP-PEST is a ubiquitously expressed cytosolic protein tyrosine phosphatase with multiple proline-rich regions that appear to be the docking sites for PTP-PEST binding partners or substrates (1). PTP-PEST regulates fibroblast adhesion, migration, and cytokinesis through its association with and dephosphorylation of p130 Cas, paxillin, PSTPIP1, WASP, and other adhesion molecules (1-5). By modulating phosphorylation states of Shc, Pyk2, Fak, and WASP, PTP-PEST negatively regulates lymphocyte activation (1,6). In mammary epithelial cells, EGF facilitates the dephosphorylation of Jak2 by PTP-PEST, thereby interfering with lactogenic hormone PRL signaling (7). PTP-PEST dephosphorylates c-Abl as well, which affects the phosphorylation states of PTP-PEST substrates such as paxillin, p130 Cas, Crk, and PSTPIP1 (8).
PTP-PEST regulates adhesion and motility of cultured epithelial cells through modulation of Rho GTPase activity (9), and is required for integrin-mediated endothelial cell adhesion and migration (10).
PTP-PEST regulates adhesion and motility of cultured epithelial cells through modulation of Rho GTPase activity (9), and is required for integrin-mediated endothelial cell adhesion and migration (10).
- Davidson, D. and Veillette, A. (2001) EMBO J 20, 3414-26.
- Garton, A.J. and Tonks, N.K. (1999) J Biol Chem 274, 3811-8.
- Shen, Y. et al. (2000) J Biol Chem 275, 1405-13.
- Angers-Loustau, A. et al. (1999) J Cell Biol 144, 1019-31.
- Côté, J.F. et al. (2002) J Biol Chem 277, 2973-86.
- Badour, K. et al. (2004) J Exp Med 199, 99-112.
- Horsch, K. et al. (2001) Mol Endocrinol 15, 2182-96.
- Cong, F. et al. (2000) Mol Cell 6, 1413-23.
- Espejo, R. et al. (2010) Am J Physiol Cell Physiol 299, C454-63.
- Souza, C.M. et al. (2012) J Biol Chem 287, 43180-90.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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