R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
PTN (F7T3F) Rabbit mAb #54934
Filter:
- WB
- IF
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 18 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Immunocytochemistry) | 1:1600 - 1:6400 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
PTN (F7T3F) Rabbit mAb recognizes endogenous levels of total PTN protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PTN protein.
Background
Pleiotrophin (PTN) is a heparin-binding, developmentally regulated cytokine that is independently identified by several groups; it is also known as heparin-binding brain mitogen (HBBM), heparin-binding growth factor-8 (HBGF-8), heparin-binding neurotrophic factor (HBNF), heparin-affinity regulatory peptide (HARP), heparin-binding growth-associated molecule (HB-GAM), and osteoblast-specific factor 1 (OSF-1) (1-6). PTN is highly upregulated during embryonic development and early cell differentiation. In adulthood, PTN expression decreases in most tissues except for the bone and the nervous system. It interacts with numerous cell surface proteoglycan and non-proteoglycan receptors, including protein tyrosine phosphatase receptor type Z (PTPRZ), syndecans, nucleolin, neuropilin-1, ALK, and integrin αVβ3 and αMβ2 (reviewed in 7-9). PTN has been associated with biological events that involve cellular proliferation, differentiation, and inflammation, including tissue regeneration, bone repair, hypoxia, tumor growth, and angiogenesis.
- Kovesdi, I. et al. (1990) Biochem Biophys Res Commun 172, 850-4.
- Li, Y.S. et al. (1990) Science 250, 1690-4.
- Merenmies, J. and Rauvala, H. (1990) J Biol Chem 265, 16721-4.
- Tezuka, K. et al. (1990) Biochem Biophys Res Commun 173, 246-51.
- Rauvala, H. (1989) EMBO J 8, 2933-41.
- Vanderwinden, J.M. et al. (1992) Anat Embryol (Berl) 186, 387-406.
- Ballesteros-Pla, C. et al. (2023) Front Endocrinol (Lausanne) 14, 1225150.
- Wang, X. (2020) Adv Clin Chem 98, 51-89.
- Deuel, T.F. et al. (2002) Arch Biochem Biophys 397, 162-71.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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