R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
PTCH1 (C53A3) Rabbit mAb #2468
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Transfected Only |
| MW (kDa) | 180-210 |
| Source/Isotype | Rabbit IgG lambda |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
PTCH1 (C53A3) Rabbit mAb detects immunoprecipitated or transfected levels of PTCH1.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro1307 of human PTCH1.
Background
Patched1 and 2 (PTCH1 and PTCH2) are twelve-pass transmembrane proteins that function as the receiving receptors for members of the Hedgehog family of proteins (1-4). In the absence of Hedgehog proteins, PTCH suppresses the otherwise constitutively active signaling receptor Smoothened (Smo) so that the Hedgehog signaling pathway is in the off state (5,6). Deactivating mutations that impair the ability of PTCH1 to suppress Smo are frequently found in patients with nevoid basal cell carcinoma syndrome (7,8). PTCH proteins have a sterol-sensing domain (SSD) also found in several proteins that function in cholesterol homeostasis, such as HMGCR (3-hydroxy-3-methylglutaryl coenzyme A-reductase) and SCAP (sterol regulatory element-binding protein-cleavage activating protein). However, the role of the SSD in Patched proteins is not clear (9,10).
PTCH1 itself is a target of Hedgehog signaling (11), with elevated PTCH1 expression as a surrogate marker for Hedgehog pathway activation (12-14).
PTCH1 itself is a target of Hedgehog signaling (11), with elevated PTCH1 expression as a surrogate marker for Hedgehog pathway activation (12-14).
- Stone, D.M. et al. (1996) Nature 384, 129-134.
- Chen, Y. and Struhl, G. (1996) Cell 87, 553-563.
- Motoyama, J. et al. (1998) Nat. Genet. 18, 104-106.
- Smyth, I. et al. (1999) Hum. Mol. Genet. 8, 291-297.
- Ingham, P.W. and McMahon, A.P. (2001) Genes Dev. 15, 3059-3087.
- McMahon, A.P. et al. (2003) Curr. Top. Dev. Biol. 53, 1-114.
- Hahn, H. et al. (1996) Cell 85, 841-851.
- Johnson, R.L. et al. (1996) Science 272, 1668-1671.
- Kuwabara, P.E. and Labouesse, M. (2002) Trends Genet. 18, 193-201.
- Chang, T.Y. et al. (2006) Annu. Rev. Cell Dev. Biol. 22, 129-157.
- Agren, M. et al. (2004) Gene 330, 101-114.
- Watkins, D.N. et al. (2003) Nature 422, 313-317.
- Berman, D.M. et al. (2003) Nature 425, 846-851.
- Karhadkar, S.S. et al. (2004) Nature 431, 707-712.
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