R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
PTBP1 (E4I3Q) Rabbit mAb #57246
Filter:
- WB
- IP
- ChIP
- eCLIP
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 57, 59 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- ChIP-Chromatin Immunoprecipitation
- eCLIP-eCLIP
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
For optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Chromatin IP | 1:50 |
| eCLIP | 1:200 |
For more information about the RBP-eCLIP service please visit Eclipsebio.
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
PTBP1 (E4I3Q) Rabbit mAb recognizes endogenous levels of total PTBP1 protein. This antibody does not cross-react with PTBP2 protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val10 of human PTBP protein.
Background
PTBP1 and PTBP2 are highly related pre-mRNA binding proteins that silence the splicing of alternative exons (1,2). While PTBP1 is widely expressed, PTBP2, also known as neural PTB or nPTB, is expressed in post-mitotic neurons. In other cell types, PTBP2 transcripts are alternatively spliced by PTBP1, which leads to nonsense-mediated decay (3). PTBP1 and 2 bind to similar regions across the transcriptome, but PTBP1 has a stronger repressive property likely due to cofactors such as Raver1 and Matrin3 (4-6). Overexpression of PTBPs in various cancers has been observed, leading to alternative splicing of key proteins in oncogenic pathways (6-9).
- Amir-Ahmady, B. et al. (2005) RNA 11, 699-716.
- Oberstrass, F.C. et al. (2005) Science 309, 2054-7.
- Boutz, P.L. et al. (2007) Genes Dev 21, 1636-52.
- Keppetipola, N.M. et al. (2016) RNA 22, 1172-80.
- Vuong, J.K. et al. (2016) Cell Rep 17, 2766-2775.
- Minami, K. et al. (2017) Oncotarget 8, 33064-33077.
- Wang, Z.N. et al. (2017) Oncotarget 8, 36185-36202.
- Uemura, Y. et al. (2017) Genes Cells 22, 785-798.
- Bielli, P. et al. (2018) Clin Cancer Res 24, 5422-5432.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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