R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
PRPF8 (F4D7U) Rabbit mAb #36884
Filter:
- WB
- IF
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 270 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Immunocytochemistry) | 1:400 - 1:800 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
PRPF8 (F4D7U) Rabbit mAb recognizes endogenous levels of total PRPF8 protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala2328 of human PRPF8 protein.
Background
Pre-mRNA-processing factor 8 (PRPF8) is the largest protein component of the human small nuclear ribonucleoprotein (snRNP) U5 and interacts with U2 and U12-dependent spliceosomes (1,2). PRPF8 forms a structure that creates a cavity for the spliceosome's catalytic center and binds to two other critical U5 components, SNRNP200 and EFTUD2. PRPF8, along with EFTUD2, affects the function of SNRNP200, an RNA helicase that unwinds the U4/U6 heterogeneous nuclear ribonucleoprotein (hnRNP) duplex to activate the spliceosome (1,3-6). snRNP U5 assembly is facilitated by HSP90 and R2TP chaperones (7). Viral RNA polymerase has been found to target PRPF8 to inhibit pre-mRNA splicing and mRNA synthesis (8). Mutations in PRPF8 resulting in inefficient SNRNP200 repression have been described in autosomal dominant retinitis pigmentosa (9,10).
- Achsel, T. et al. (1998) Mol Cell Biol 18, 6756-66.
- Luo, H.R. et al. (1999) RNA 5, 893-908.
- Mozaffari-Jovin, S. et al. (2013) Science 341, 80-4.
- Kuhn, A.N. et al. (1999) Mol Cell 3, 65-75.
- Small, E.C. et al. (2006) Mol Cell 23, 389-99.
- Maeder, C. et al. (2009) Nat Struct Mol Biol 16, 42-8.
- Malinová, A. et al. (2017) J Cell Biol 216, 1579-1596.
- Liu, Y.C. et al. (2014) PLoS Pathog 10, e1004199.
- Martínez-Gimeno, M. et al. (2003) Invest Ophthalmol Vis Sci 44, 2171-7.
- van Lith-Verhoeven, J.J. et al. (2002) Ophthalmic Genet 23, 1-12.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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