R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
PRDX6 (D9J9H) Rabbit mAb #64329
Filter:
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 25 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
PRDX6 (D9J9H) Rabbit mAb recognizes endogenous levels of total PRDX6 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human PRDX6 protein.
Background
Peroxiredoxin 6 (PRDX6) belongs to an antioxidant enzyme family of non-seleno peroxidases. PRDX6 is a unique member of the PRDX family, exhibiting both glutathione peroxidase and phospholipase A2 activities (1,2). PRDX6 regulates phospholipid turnover in addition to protecting cells against oxidative injury. PRDX6 is expressed in all major organs, with a particularly high level in lung, where it regulates lung surfactant phospholipid synthesis and turnover (3-5). Research studies have shown that PRDX6 is aberrantly expressed in various cancers, and can promote cancer cell metastasis and invasion (6,7). Elevated expression of PRDX6 and related PRDX family members has also been shown to contribute to drug resistance in cancer cells (8,9). PRDX6 is also expressed in neutrophils, where it has been shown to activate NADPH oxidase (NOX2) (10-12).
- Kang, S.W. et al. (1998) J Biol Chem 273, 6303-11.
- Chen, J.W. et al. (2000) J Biol Chem 275, 28421-7.
- Manevich, Y. and Fisher, A.B. (2005) Free Radic Biol Med 38, 1422-32.
- Schremmer, B. et al. (2007) Subcell Biochem 44, 317-44.
- Lee, S.B. et al. (2009) Mol Cells 28, 583-8.
- Ho, J.N. et al. (2010) Mol Cancer Ther 9, 825-32.
- Yun, H.M. et al. (2014) Free Radic Biol Med 69, 367-76.
- Pak, J.H. et al. (2011) Cancer Invest 29, 21-8.
- Kalinina, E.V. et al. (2012) Bull Exp Biol Med 153, 878-81.
- Chatterjee, S. et al. (2011) J Biol Chem 286, 11696-706.
- Ambruso, D.R. et al. (2012) Biochim Biophys Acta 1823, 306-15.
- Ambruso, D.R. (2013) Methods Enzymol 527, 145-67.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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