R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
PRDM16 (F3K9T) Rabbit mAb #16212
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 170 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
PRDM16 (F3K9T) Rabbit mAb recognizes endogenous levels of total PRDM16 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro666 of human PRDM16 protein.
Background
PRDM16 is a transcription factor that is structurally characterized by a conserved N-terminal PRDI-BF1 and RIZ1 homology (PR) domain and a variable number of zinc fingers (1,2). The PR domain bears similarities to the catalytic suppressor of variegation 3–9, enhancer of zeste, and trithorax (SET) domain that is found in a group of histone methyltransferases (1,3). Indeed, PRDM16 has been shown to catalyze mono-methylation of histone 3 at lysine (H3K9me1) (4). Human PRDM16 encodes a protein with four isoforms with the most extensively studied isoforms being the full-length PRDM16 and the PR-lacking isoform that is generated by a transcript derived from an internal promoter (5). PRDM16 is involved in the regulation of various biological processes, including adipose biology and energy homeostasis. This protein is also expressed in hematopoietic stem cells (HSCs) and plays a key role in the establishment and maintenance of these cells (6,7). More recently, PRDM16 was shown to be a suppressor of lung adenocarcinoma metastasis, and its low expression predicted poor prognosis of lung adenocarcinoma patients (8).
- Hohenauer, T. and Moore, A.W. (2012) Development 139, 2267-82.
- Fog, C.K. et al. (2012) Bioessays 34, 50-60.
- Di Zazzo, E. et al. (2013) Biology (Basel) 2, 107-41.
- Pinheiro, I. et al. (2012) Cell 150, 948-60.
- Nishikata, I. et al. (2003) Blood 102, 3323-32.
- Yoshida, M. et al. (2004) Blood 103, 2753-60.
- Yu, H. et al. (2014) Blood 124, 1737-47.
- Fei, L.R. et al. (2019) J Exp Clin Cancer Res 38, 35.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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