R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
PRDM14 (E1D5S) XP® Rabbit mAb #83527
Filter:
- WB
- IP
- IF
- F
- ChIP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 70 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
- F-Flow Cytometry
- ChIP-Chromatin Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
For optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunofluorescence (Immunocytochemistry) | 1:400 |
| Flow Cytometry (Fixed/Permeabilized) | 1:800 |
| Chromatin IP | 1:50 |
| Chromatin IP-seq | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
PRDM14 (E1D5S) XP® Rabbit mAb recognizes endogenous levels of total PRDM14 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with full-length recombinant protein specific to human PRDM14 protein.
Background
PR domain zinc finger protein 14 (PRDM14) is a likely protein lysine methyltransferase that is primarily expressed in primordial germ cells and pluripotent embryonic stem cells. It is essential for the establishment and maintenance of primordial germ cells and critical for the maintenance of pluripotency in embryonic stem cells (1-3). PRDM14 represses genes involved in the differentiation of stem cells into various cell lineages, likely via a combination of interactions with TET proteins, the polycomb repressive complex 2 (PRC2), and CBFA2T2 (3-8). In addition, overexpression of PRDM14 in combination with Jarid2 promotes induced pluripotent stem cell (iPSC) formation (9). PRDM14 protein levels are overexpressed in certain cancers, including breast, leukemia (T-ALL), and non-small cell lung cancer (NSCLC) (10-13), and PRDM14 overexpression may serve as a novel prognostic marker in NSCLC (14). Targeting PRDM14 overexpression with a siRNA-based therapy was shown to decrease liver metastasis in a murine pancreatic cancer model, suggesting potential as a therapeutic option for cancers where this protein is abnormally expressed (15).
- Yamaji, M. et al. (2008) Nat Genet 40, 1016-22.
- Chia, N.Y. et al. (2010) Nature 468, 316-20.
- Tsuneyoshi, N. et al. (2008) Biochem Biophys Res Commun 367, 899-905.
- Ma, Z. et al. (2011) Nat Struct Mol Biol 18, 120-7.
- Okashita, N. et al. (2014) Development 141, 269-80.
- Yamaji, M. et al. (2013) Cell Stem Cell 12, 368-82.
- Nady, N. et al. (2015) Elife 4, e10150.
- Tu, S. et al. (2016) Nature 534, 387-90.
- Iseki, H. et al. (2016) Stem Cells 34, 322-33.
- Moelans, C.B. et al. (2010) Mod Pathol 23, 1029-39.
- Nishikawa, N. et al. (2007) Cancer Res 67, 9649-57.
- Carofino, B.L. et al. (2013) Dis Model Mech 6, 1494-506.
- Liu, B. et al. (2010) Zhongguo Fei Ai Za Zhi 13, 867-72.
- Zhang, T. et al. (2013) Med Oncol 30, 605.
- Moriya, C. et al. (2017) Carcinogenesis 38, 638-648.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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