PRC1 Antibody #3639
Filter:
- WB
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 70 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
PRC1 Antibody recognizes endogenous levels of total PRC1 protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly522 of human PRC1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Protein Regulator of Cytokinesis 1 (PRC1) is a member of the MAP65/ASE1 family of nonmotor microtubule-associated proteins, first described in budding yeast (1,2). PRC1 is a substrate of CDK1, which maintains PRC1 in an inactive, monomeric state (2). Cell-cycle dependent degradation of CDK1 leads to dephosphorylation of PRC1 and subsequent KIF4-mediated translocation to the plus ends of microtubules, where it promotes microtubule bundling by cross-linking antiparallel microtubules (3-5). The microtubule bundling functions of PRC1 play a critical role in maintaining structural integritry of the spindle midzone during cytokinesis (3,6,7).
- Pellman, D. et al. (1995) J Cell Biol 130, 1373-85.
- Jiang, W. et al. (1998) Mol Cell 2, 877-85.
- Kurasawa, Y. et al. (2004) EMBO J 23, 3237-48.
- Zhu, C. and Jiang, W. (2005) Proc Natl Acad Sci USA 102, 343-8.
- Subramanian, R. et al. (2010) Cell 142, 433-43.
- Mollinari, C. et al. (2002) J Cell Biol 157, 1175-86.
- Zhu, C. et al. (2006) Proc Natl Acad Sci USA 103, 6196-201.
限制使用
除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受， 否书均被拒书，并且无效。
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For Research Use Only. Not For Use In Diagnostic Procedures.
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