R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
PQBP1 (E5N7J) Rabbit mAb #17134
Filter:
- WB
- IP
- IF
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 33 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunofluorescence (Immunocytochemistry) | 1:100 - 1:400 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
PQBP1 (E5N7J) Rabbit mAb recognizes endogenous levels of total PQBP1 protein. This antibody cross-reacts with a 50 kDa protein of unknown identity in mouse models by western blot. Mouse species reactivity is western blot only.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PQBP1 protein.
Background
Polyglutamine binding protein 1 (PQBP1) is an intrinsically disordered protein that is broadly expressed and evolutionarily conserved (1-3). PQBP1 has primarily been detected in the nucleus, though it has also been observed in the cytoplasm of mouse microglia (4,5). In the nucleus, PQBP1 is known to interact with mRNA splicing and RNA binding proteins, as well as the polyglutamine protein ataxin-1, and plays roles in both transcription and translation (4, 6-8). Mutations leading to loss of function of PQBP1 are associated with Golabi-Ito-Hall syndrome and Renpenning's syndrome, hallmarks of which include microcephaly and intellectual disability (9-11). Recently, PQBP1 has been reported to interact with tau protein, mediating activation of the cGAS-STING pathway in microglia (5). In the context of HIV-1 infection, PQBP1 also activates the cGAS-STING pathway by binding to reverse-transcribed HIV-1 DNA and cGAS, resulting in an innate immune response driven by IRF-3 (12).
- Takahashi, M. et al. (2009) Biochim Biophys Acta 1794, 936-43.
- Waragai, M. et al. (1999) Hum Mol Genet 8, 977-87.
- Okazawa, H. et al. (2001) Brain Res Bull 56, 273-80.
- Okazawa, H. et al. (2002) Neuron 34, 701-13.
- Jin, M. et al. (2021) Nat Commun 12, 6565.
- Mizuguchi, M. et al. (2014) Nat Commun 5, 3822.
- Llorian, M. et al. (2004) Biochem J 378, 229-38.
- Shen, Y. et al. (2021) Mol Cell 81, 1425-1438.e10.
- Stevenson, R.E. et al. (2005) Am J Med Genet A 134, 415-21.
- Lubs, H. et al. (2006) J Med Genet 43, e30.
- Kalscheuer, V.M. et al. (2003) Nat Genet 35, 313-5.
- Yoh, S.M. et al. (2015) Cell 161, 1293-1305.
限制使用
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