R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
PPARδ (E8O3H) Rabbit mAb #74076
Filter:
- WB
- IP
- ChIP
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 50 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- ChIP-Chromatin Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Chromatin IP | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
PPARδ (E8O3H) Rabbit mAb recognizes endogenous levels of total PPARδ protein. This antibody may cross-react with overexpressed PPARα protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant protein fragment specific to human PPARδ protein.
Background
Peroxisome proliferator-activated receptor-δ (PPARδ, also known as PPARβ or PPARβ/δ) is a widely expressed member of the PPAR nuclear receptor family, which controls lipid homeostasis (1,2). In response to various ligands, PPAR proteins heterodimerize with retinoid X receptors (RXRs) in order to bind DNA and regulate target genes (3,4). PPARδ plays a role in many different biological functions, including cholesterol efflux, embryo implantation, preadipocyte proliferation, and wound healing (5-8). PPARδ has been implicated in colorectal cancer (CRC), as it is normally downregulated by APC, a tumor suppressor frequently knocked out in CRCs (9). More recently, high fat diets have been found to induce PPARδ in intestinal stem cells and progenitors, increasing their tumorigenicity (10). Furthermore, in Huntington's disease (HD) mouse models, it was shown that PPARδ was unable bind to huntingtin protein when mutated, which repressed its function. Agonist-induced activation of PPARδ in HD model mice improved cognitive function and increased survival time (11).
- Kliewer, S.A. et al. (1994) Proc Natl Acad Sci U S A 91, 7355-9.
- Braissant, O. et al. (1996) Endocrinology 137, 354-66.
- Miyata, K.S. et al. (1994) Gene 148, 327-30.
- Forman, B.M. et al. (1997) Proc Natl Acad Sci U S A 94, 4312-7.
- Oliver, W.R. et al. (2001) Proc Natl Acad Sci U S A 98, 5306-11.
- Lim, H. et al. (1999) Genes Dev 13, 1561-74.
- Hansen, J.B. et al. (2001) J Biol Chem 276, 3175-82.
- Tan, N.S. et al. (2001) Genes Dev 15, 3263-77.
- He, T.C. et al. (1999) Cell 99, 335-45.
- Beyaz, S. et al. (2016) Nature 531, 53-8.
- Dickey, A.S. et al. (2016) Nat Med 22, 37-45.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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