R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
PODXL (E8O1S) Rabbit mAb (BSA and Azide Free) #66798
Filter:
- WB
- IHC
- IF
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 200 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
This product is the carrier free version of product #55601. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.
Formulation
Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.
For standard formulation of this product see product #55601
For standard formulation of this product see product #55601
Storage
Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.
Specificity / Sensitivity
PODXL (E8O1S) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total PODXL protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human PODXL protein.
Background
Podocalyxin is an O-glycosylated and sialylated membrane protein that is closely similar to CD34, the well characterized marker for hematopoietic stem cells (1). In association with several intracellular binding partners, podocalyxin contributes to adhesion and cell morphology (2-4). Podocalyxin is expressed in hematopoietic tissues during stages of active hematopoietic expansion in embryonic development and in more restricted, mainly megakaryocytic lineages in the adult (1,4). Expression also occurs in kidney podocytes, both in embryo and adult, where podocalyxin is essential for organ development and proper kidney function (5-7). A few other cell types that express podocalyxin include some specialized vascular endothelia (8,9) and a subset of neurons (10). It is also aberrantly expressed in a diverse number of cancers, including breast, testicular, pancreatic cancers, and in leukemia (11). Expression is often correlated with poor outcome (11).
- Doyonnas, R. et al. (2005) Blood 105, 4170-8.
- Donowitz, M. et al. (2005) J Physiol 567, 3-11.
- Tan, P.C. et al. (2006) Stem Cells 24, 1150-61.
- Sathyanarayana, P. et al. (2007) Blood 110, 509-18.
- Orlando, R.A. et al. (2001) J Am Soc Nephrol 12, 1589-98.
- Kerjaschki, D. et al. (1984) J Cell Biol 98, 1591-6.
- Doyonnas, R. et al. (2001) J Exp Med 194, 13-27.
- Sassetti, C. et al. (1998) J Exp Med 187, 1965-75.
- Baumhueter, S. et al. (1994) Blood 84, 2554-65.
- Vitureira, N. et al. (2010) PLoS One 5, e12003.
- Nielsen, J.S. and McNagny, K.M. (2009) J Am Soc Nephrol 20, 1669-76.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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