Render Target: SSR
Render Timestamp: 2024-12-19T21:38:13.656Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-09-30 01:57:18.618
Product last modified at: 2024-12-17T19:01:49.794Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

PNUTS (D8H5J) Rabbit mAb #80920

Filter:
  • WB

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 115
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    PNUTS (D8H5J) Rabbit mAb recognizes endogenous levels of total PNUTS protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys244 of human PNUTS protein.

    Background

    Protein phosphatase-1 nuclear targeting subunit (PNUTS) is one of the key regulators of protein phosphatase 1 (PP1) in the nucleus (1). Via interaction with PP1, PNUTS plays an essential role in multiple cellular processes, including chromatin decondensation (2), DNA damage response (3), and cardiomyocyte apoptosis (4). Notably, PNUTS also regulates the activity of two key tumor suppressors, Rb and p53, through inhibition of PP1 mediated dephosphorylation (5-7). Research studies indicate that PNUTS also sequesters PTEN in the nucleus through direct interaction and inhibits its tumor suppressor function (8). PNUTS is ubiquitously expressed and elevated PNUTS expression is observed in various cancers such as esophageal carcinoma, squamous cell carcinoma, and prostate cancer (1,8).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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