PNUTS Antibody #14171
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 115 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
PNUTS Antibody recognizes endogenous levels of total PNUTS protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val380 of human PNUTS protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Protein phosphatase-1 nuclear targeting subunit (PNUTS) is one of the key regulators of protein phosphatase 1 (PP1) in the nucleus (1). Via interaction with PP1, PNUTS plays an essential role in multiple cellular processes, including chromatin decondensation (2), DNA damage response (3), and cardiomyocyte apoptosis (4). Notably, PNUTS also regulates the activity of two key tumor suppressors, Rb and p53, through inhibition of PP1 mediated dephosphorylation (5-7). Research studies indicate that PNUTS also sequesters PTEN in the nucleus through direct interaction and inhibits its tumor suppressor function (8). PNUTS is ubiquitously expressed and elevated PNUTS expression is observed in various cancers such as esophageal carcinoma, squamous cell carcinoma, and prostate cancer (1,8).
- Allen, P.B. et al. (1998) J Biol Chem 273, 4089-95.
- Landsverk, H.B. et al. (2005) Biochem J 390, 709-17.
- Landsverk, H.B. et al. (2010) EMBO Rep 11, 868-75.
- Boon, R.A. et al. (2013) Nature 495, 107-10.
- Lee, S.J. et al. (2007) Cell Death Differ 14, 1106-16.
- De Leon, G. et al. (2008) Cancer Biol Ther 7, 833-41.
- Choy, M.S. et al. (2014) Proc Natl Acad Sci U S A 111, 4097-102.
- Kavela, S. et al. (2013) Cancer Res 73, 205-14.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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