R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
PNPLA3 (E7N6O) Rabbit mAb #95546
Filter:
- WB
- IHC
Supporting Data
| REACTIVITY | M |
| SENSITIVITY | Endogenous (IHC-P), Transfected (W) |
| MW (kDa) | 58 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunohistochemistry (Paraffin) | 1:150 - 1:600 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #49662.
For a carrier free (BSA and azide free) version of this product see product #49662.
Protocol
Specificity / Sensitivity
PNPLA3 (E7N6O) Rabbit mAb recognizes transfected levels of total PNPLA3 protein by western blot and endogenous levels of total PNPLA3 protein by immunohistochemistry.
Species Reactivity:
Mouse
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val184 of murine PNPLA3 protein.
Background
PNPLA3, also known as adiponutrin, is a protein encoded by the PNPLA3 gene. It is a member of the patatin-like phospholipase domain (PNPLA) family of proteins, which are involved in various lipid metabolic processes. PNPLA3 is expressed primarily in the liver and adipose tissue, where it is localized to lipid droplets (1). PNPLA3 is also associated with several diseases, including nonalcoholic fatty liver disease (NAFLD). NAFLD is a spectrum of liver diseases that can range from simple steatosis (fatty liver) to nonalcoholic steatohepatitis (NASH), cirrhosis, and hepatocellular carcinoma (HCC) (2). PNPLA3 is the strongest genetic risk factor for NAFLD and its associated complications (3). Mouse PNPLA3 is a valuable tool for studying the role of PNPLA3 in human health and disease (4).
限制使用
除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受， 否书均被拒书，并且无效。
专品专有“专供研究使用”的专专或专似的专专声明， 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的， 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的， 或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专
For Research Use Only. Not For Use In Diagnostic Procedures.
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