PMEL/Melanoma gp100 (HMB45) Mouse mAb #38815
Filter:
- IHC
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Mouse IgG1 kappa |
Application Key:
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| IHC Leica Bond | 1:800 - 1:3200 |
| Immunohistochemistry (Paraffin) | 1:400 - 1:1600 |
Storage
Supplied in 10 mM PBS containing 0.05% BSA and 0.05% sodium azide. Stable for 36 months when stored at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present, but will not interfere with antibody performance.
Protocol
Specificity / Sensitivity
PMEL/Melanoma gp100 (HMB45) Mouse mAb recognizes endogenous levels of total PMEL/Melanoma gp100 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with extract of pigmented melanoma metastases from lymph nodes.
Background
PMEL/Melanoma gp100 is a type 1 transmembrane glycoprotein primarily expressed in pigment cells of the skin and eye, and overexpressed in more than 75% of human melanomas (1-3). It is involved in the transformation of melanosomes from stage I to stage II, forming the fibrillar matrix necessary for pigmentation of the cell (4). The synthesis and modification of PMEL/Melanoma gp100 is done in the endoplasmic reticulum (ER) and transferred to the Golgi complex where it is cleaved into two disulfide-linked subunits, Mα and Mβ. The cleavage of the large luminal Mα fragment is required for the formation of the fibrils that are the structure for melanin synthesis (5,6). The ability for PMEL/Melanoma gp100 to form functional amyloids makes it an interesting protein to study when looking at pathological amyloids in neurodegenerative diseases, such as Alzheimer’s disease and Parkinson’s disease (5,7). Due to melanocyte-restricted expression of this protein, PMEL/Melanoma gp100 is considered a tumor-associated antigen that can be targeted by immunotherapeutic approaches to treat melanoma (8-10).
- Theos, A.C. et al. (2005) Pigment Cell Res 18, 322-36.
- Cormier, J.N. et al. (1998) J Immunother 21, 27-31.
- Robila, V. et al. (2008) J Immunol 181, 7843-52.
- Hoashi, T. et al. (2006) J Biol Chem 281, 21198-208.
- Bissig, C. et al. (2016) Int J Mol Sci 17, 1438.
- Berson, J.F. et al. (2001) Mol Biol Cell 12, 3451-64.
- van Niel, G. (2016) Cell Mol Neurobiol 36, 327-42.
- Schwartzentruber, D.J. et al. (2011) N Engl J Med 364, 2119-27.
- Baba, T. et al. (2010) J Transl Med 8, 84.
- Lesterhuis, W.J. et al. (2011) Cancer Immunol Immunother 60, 249-60.
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