R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
PLSCR1 (E4Z8R) Rabbit mAb #55260
Filter:
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 35, 45-100 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
PLSCR1 (E4Z8R) Rabbit mAb recognizes endogenous levels of total PLSCR1 protein. In addition to the predicted 35 kDa band observed, PLSCR1 (E4Z8R) Rabbit mAb detects multiple bands spanning 45-100 kDa representing post-translationally modified PLSCR1, including lipidated forms of the target protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human PLSCR1 protein.
Background
Phospholipid scramblase 1 (PLSCR1) is 1 of 4 scramblases encoded in the human genome (1). Phospholipid scramblases function to catalyze calcium-mediated ATP-independent mobilization of phospholipids between the inner and outer leaflet of plasma membrane in activated, injured, or apoptotic cells. PLSCR1 is a type-II transmembrane protein with a short 9 amino acid C-terminal domain and a long N-terminal extracellular domain. Although PLSCR1 is part of a family of proteins that function to catalyze phospholipid mobilization, PLSCR1 may have alternative functions (2,3). Rather, PLSCR1 may function to activate cell signaling pathways, particularly in phagocytic cells of monocyte/macrophage lineage (4). Microglia, resident macrophages of the brain, play a key role in clearance of cellular and pathologic debris where altered phagocytosis may contribute to neurodegenerative diseases like Alzheimer’s disease. PLSCR1 may regulate microglia phagocytosis, representing a potential therapeutic target to modulate microglia phagocytosis (5).
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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