Render Target: SSR
Render Timestamp: 2025-02-25T03:26:07.846Z
Commit: 7500bcdc731e9059bbdfbdbe9e72caa896e426e8
XML generation date: 2024-09-30 01:58:12.424
Product last modified at: 2025-01-01T09:03:12.816Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

PLSCR1 (E4Z8R) Rabbit mAb #55260

Filter:
  • WB
Western Blotting Image 1: PLSCR1 (E4Z8R) Rabbit mAb
Western blot analysis of extracts from various cell lines using PLSCR1 (E4Z8R) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).

To Purchase # 55260

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 35, 45-100
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • H-Human 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

PLSCR1 (E4Z8R) Rabbit mAb recognizes endogenous levels of total PLSCR1 protein. In addition to the predicted 35 kDa band observed, PLSCR1 (E4Z8R) Rabbit mAb detects multiple bands spanning 45-100 kDa representing post-translationally modified PLSCR1, including lipidated forms of the target protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human PLSCR1 protein.

Background

Phospholipid scramblase 1 (PLSCR1) is 1 of 4 scramblases encoded in the human genome (1). Phospholipid scramblases function to catalyze calcium-mediated ATP-independent mobilization of phospholipids between the inner and outer leaflet of plasma membrane in activated, injured, or apoptotic cells. PLSCR1 is a type-II transmembrane protein with a short 9 amino acid C-terminal domain and a long N-terminal extracellular domain. Although PLSCR1 is part of a family of proteins that function to catalyze phospholipid mobilization, PLSCR1 may have alternative functions (2,3). Rather, PLSCR1 may function to activate cell signaling pathways, particularly in phagocytic cells of monocyte/macrophage lineage (4). Microglia, resident macrophages of the brain, play a key role in clearance of cellular and pathologic debris where altered phagocytosis may contribute to neurodegenerative diseases like Alzheimer’s disease. PLSCR1 may regulate microglia phagocytosis, representing a potential therapeutic target to modulate microglia phagocytosis (5).
For Research Use Only. Not For Use In Diagnostic Procedures.
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