R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
PLK4 (E6A7R) Rabbit mAb #71033
Filter:
- WB
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 105 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
PLK4 (E6A7R) Rabbit mAb recognizes endogenous levels of total PLK4 protein. An unknown band at 170 kDa has been observed.
Species Reactivity:
Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu678 of human PLK4 protein.
Background
At least four distinct polo-like kinases exist in mammalian cells: PLK1, PLK2, PLK3, and PLK4/SAK (1). PLK4/SAK is transcriptionally repressed by p53 and may contribute to p53-mediated apoptosis (2). PLK4 has also been identified as a key regulator of centriole duplication (3-5). PLK4 is typically expressed at low levels and is controlled by autoregulated instability such that treatment with PLK4 inhibitors, such as CFI-400945, leads to an increase in PLK4 expression (6,7). PLK4 is aberrantly expressed in multiple cancers and has emerged as a potential prognostic and therapeutic target (8).
- Nigg, E.A. (1998) Curr Opin Cell Biol 10, 776-83.
- Li, J. et al. (2005) Neoplasia 7, 312-23.
- Habedanck, R. et al. (2005) Nat Cell Biol 7, 1140-6.
- Ko, M.A. et al. (2005) Nat Genet 37, 883-8.
- Bettencourt-Dias, M. et al. (2005) Curr Biol 15, 2199-207.
- Holland, A.J. et al. (2012) Genes Dev 26, 2684-9.
- Mason, J.M. et al. (2014) Cancer Cell 26, 163-76.
- Zhao, Y. and Wang, X. (2019) J Cancer Res Clin Oncol 145, 2413-22.
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