R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
PLK2 (D5R2B) Rabbit mAb #14812
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 70 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
PLK2 (D5R2B) Rabbit mAb recognizes endogenous levels of total PLK2 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro67 of human PLK2 protein.
Background
At least five distinct polo-like kinases exist in mammalian cells: PLK1, PLK2, PLK3, PLK4/SAK, and the non-catalytic PLK5 protein (1). The p53-induced PLK2 functions in centriole duplication, as well as at spindle and S phase checkpoints (3-5). Research studies show that PLK2 expression is related to chemosensitivity in ovarian cancer. Downregulation of PLK2 expression in chemosensitive ovarian cancer cells is associated with a greater chance of relapse in patients following specific treatment regimens (6). PLK2 can phosphorylate α-synuclein at Ser129, which is a site shown to be involved in diseases of the central nervous system (7,8). Polo-like kinase 2 also phosphorylates GEFs and GAPs, regulating Ras and Rap small GTPase function in neurons (9).
- de Cárcer, G. et al. (2011) Cell Cycle 10, 2255-62.
- Cizmecioglu, O. et al. (2008) Cell Cycle 7, 3548-55.
- Burns, T.F. et al. (2003) Mol Cell Biol 23, 5556-71.
- Matthew, E.M. et al. (2007) Cell Cycle 6, 2571-8.
- Cizmecioglu, O. et al. (2012) J Cell Sci 125, 981-92.
- Syed, N. et al. (2011) Cancer Res 71, 3317-27.
- Inglis, K.J. et al. (2009) J Biol Chem 284, 2598-602.
- Bergeron, M. et al. (2014) Neuroscience 256, 72-82.
- Lee, K.J. et al. (2011) Neuron 69, 957-73.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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