PKD/PKCμ Antibody #2052
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Supporting Data
| REACTIVITY | H M Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 115 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Flow Cytometry (Fixed/Permeabilized) | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
PKD/PKCmu Antibody detects endogenous levels of total PKD1/PKCmu protein. This antibody may also react with isoform PKD2, in some species.
Species Reactivity:
Human, Mouse, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser916 of mouse PKD. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Activation of PKC is one of the earliest events in a cascade leading to a variety of cellular responses, such as secretion, gene expression, proliferation, and muscle contraction (1,2). Protein kinase D (PKD), also called PKCμ, is a serine/threonine kinase whose activation is dependent on the phosphorylation of two activation loop sites, Ser744 and Ser748, via a PKC-dependent signaling pathway (3-5). In addition to the two activation loop sites, the carboxy-terminal Ser916 has been identified as an autophosphorylation site for PKD/PKCμ. Phosphorylation at Ser916 correlates with PKD/PKCμ catalytic activity (6).
- Nishizuka, Y. (1984) Nature 308, 693-698.
- Keranen, L.M. (1995) Curr. Biol. 5, 1394-1403.
- Valverde, A.M. et al. (1994) Proc. Natl. Acad. Sci. 91, 8572-8576.
- Johannes, F.J. et al. (1994) J. Biol. Chem. 269, 6140-6148.
- Iglesias, T. et al. (1998) J. Biol. Chem. 273, 27662-27667.
- Matthews, S.A. et al. (1999) J. Biol. Chem. 274, 26543-26549.
- Wood, C.D. et al. (2005) J Biol Chem 280, 6245-51.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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