R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
PICK1 (D6W7M) Rabbit mAb #85325
Filter:
- WB
- IP
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing MycDDK-tagged full-length human PICK1 protein (hPICK1-MycDDK; +), using PICK1 (D6W7M) Rabbit mAb.
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 50 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Related Products
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
PICK1 (D6W7M) Rabbit mAb recognizes endogenous levels of total PICK1 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro20 of human PICK1 protein.
Background
PICK1, or Protein interacting with C-kinase 1, is a cytosolic adaptor protein composed of an N-terminus PDZ domain and a C-terminus BAR domain that allow protein and membrane interactions, respectively (1,2). PICK1 regulates endosomal trafficking and surface expression of AMPA receptors and is therefore involved in synaptic plasticity (3). PICK1 is a negative regulator of Arp2/3-dependent actin polymerization and also for the development of neuronal architecture (4). Finally, increasing evidence indicates that PICK1 expression is upregulated in a number cancers (5), and that PICK1 interacts with proteins involved in the promotion of tumorigenesis such as Ephrin receptors, Coxsackie-adenovirus receptor (CAR), and ErbB2/Her2 (6,7).
- Staudinger, J. et al. (1995) J Cell Biol 128, 263-71.
- Jin, W. et al. (2006) J Neurosci 26, 2380-90.
- Xia, J. et al. (1999) Neuron 22, 179-87.
- Rocca, D.L. et al. (2008) Nat Cell Biol 10, 259-71.
- Zhang, B. et al. (2010) Cancer Sci 101, 1536-42.
- Jaulin-Bastard, F. et al. (2001) J Biol Chem 276, 15256-63.
- Excoffon, K.J. et al. (2004) J Cell Sci 117, 4401-9.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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