R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
PI3 Kinase p110β (C33D4) Rabbit mAb #3011
Filter:
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 110 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:10 - 1:50 |
| Immunoprecipitation | 1:25 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
PI3 Kinase p110β (C33D4) Rabbit mAb detects endogenous levels of total PI3K p110β protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide of human PI3K p110β.
Background
Phosphoinositide 3-kinase (PI3K) catalyzes the production of phosphatidylinositol-3,4,5-triphosphate by phosphorylating phosphatidylinositol (PI), phosphatidylinositol-4-phosphate (PIP), and phosphatidylinositol-4,5-bisphosphate (PIP2). Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration, and cell survival (1). PTEN reverses this process, and research studies have shown that the PI3K signaling pathway is constitutively activated in human cancers that have loss of function of PTEN (2). PI3Ks are composed of a catalytic subunit (p110) and a regulatory subunit. Various isoforms of the catalytic subunit (p110α, p110β, p110γ, and p110δ) have been isolated, and the regulatory subunits that associate with p110α, p110β, and p110δ are p85α and p85β (3). In contrast, p110γ associates with a p101 regulatory subunit that is unrelated to p85. Furthermore, p110γ is activated by βγ subunits of heterotrimeric G proteins (4).
p110ß is widely distributed in tissue and plays an essential role in early embryonic development (5). p110ß stimulates cell proliferation, invasive cell growth, and expression is increased in a number of tumors including glioblastomas (6-8).
p110ß is widely distributed in tissue and plays an essential role in early embryonic development (5). p110ß stimulates cell proliferation, invasive cell growth, and expression is increased in a number of tumors including glioblastomas (6-8).
- Cantley, L.C. (2002) Science 296, 1655-7.
- Simpson, L. and Parsons, R. (2001) Exp Cell Res 264, 29-41.
- Neri, L.M. et al. (2002) Biochim Biophys Acta 1584, 73-80.
- Stoyanov, B. et al. (1995) Science 269, 690-3.
- Okkenhaug, K. and Vanhaesebroeck, B. (2003) Nat Rev Immunol 3, 317-30.
- Czauderna, F. et al. (2003) Nucleic Acids Res 31, 670-82.
- Bénistant, C. et al. (2000) Oncogene 19, 5083-90.
- Knobbe, C.B. and Reifenberger, G. (2003) Brain Pathol 13, 507-18.
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