R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
PI3 Kinase Class II α (D3Q5B) Rabbit mAb #12402
Filter:
- WB
- IP
Supporting Data
REACTIVITY | H M R Mk |
SENSITIVITY | Endogenous |
MW (kDa) | 180 |
Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:50 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
PI3 Kinase Class II α (D3Q5B) Rabbit mAb recognizes endogenous levels of total PI3K class II α protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly717 of human PI3K class II α protein.
Background
Class II phosphatidylinositol 3-kinases (PI3K) contain a C-terminal C2 domain that is unique to the class II isoforms of the PI3K family. This C2 domain mediates protein and phospholipid binding acitivities (1,2). PI3K Class II α generates phosphatidylinositol 3-phosphate (PIP3) and phosphatidylinositol 3,4-bisphosphate (PI(3, 4)P2) from phosphatidylinositol and phosphatidylinositol 4-phosphate (3). PI3K Class II α is located in various intracellular locations such as the trans-Golgi network, endocytic compartments, clathrin-coated vesicles, and nuclear speckles (1,4,5). Research studies have indicated that PI3K Class II α regulates the assembly and distribution of clathrin, resulting in the modulation of clathrin-dependent trafficking and sorting within the trans Golgi network (5,6). PI3K Class II α also mediates translocation of the glucose transporter GLUT4 to the plasma membrane in response to insulin (7). PI3K Class II α has also been shown to regulate neurosecretory granule exocytosis (8) and vascular smooth muscle contraction (9). Unlike other PI3K family members, PI3K Class II α is less sensitive to the PI3K inhibitors wortmannin and LY294002 (3).
- Didichenko, S.A. and Thelen, M. (2001) J Biol Chem 276, 48135-42.
- Stahelin, R.V. et al. (2006) J Biol Chem 281, 39396-406.
- Domin, J. et al. (1997) Biochem J 326 ( Pt 1), 139-47.
- Domin, J. et al. (2000) J Biol Chem 275, 11943-50.
- Gaidarov, I. et al. (2001) Mol Cell 7, 443-9.
- Gaidarov, I. et al. (2005) J Biol Chem 280, 40766-72.
- Falasca, M. et al. (2007) J Biol Chem 282, 28226-36.
- Wen, P.J. et al. (2008) Mol Biol Cell 19, 5593-603.
- Yoshioka, K. et al. (2007) Mol Pharmacol 71, 912-20.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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